Yuav kom ntsuam xyuas qhov cuam tshuam ntawm kev ntxiv triglyceride rau hauv cov zaub mov rau kev tsim cov protein microbial hauv rumen thiab cov yam ntxwv ntawm kev fermentation ntawm cov yaj me me laus, ob qho kev sim tau ua nyob rau hauv vitro thiab hauv vivo.

Kev sim hauv vitro: cov khoom noj khoom haus basal (raws li cov khoom qhuav) nrog triglyceride concentration ntawm 0, 2, 4, 6 thiab 8g / kg tau siv ua lub substrate, thiab cov kua txiv rumen ntawm Adult Small Tailed ewes tau ntxiv, thiab incubated ntawm 39 ℃ rau 48h hauv vitro.

Kev sim hauv lub cev: 45 tus yaj laus tau muab faib ua 5 pawg raws li lawv qhov hnyav pib (55 ± 5 kg).Glyceryl tributylatentawm 0, 2, 4, 6 thiab 8 g/kg (raws li cov khoom qhuav) tau ntxiv rau hauv cov khoom noj tseem ceeb, thiab cov kua dej thiab zis ntawm cov rumen tau sau rau 18 hnub.

Cov txiaj ntsig ntawm kev xeem

1). Cov nyhuv rau tus nqi pH thiab cov roj fatty acid concentration

Cov txiaj ntsig tau qhia tias tus nqi pH ntawm cov kab lis kev cai txo qis linearly thiab qhov concentration ntawm tag nrho cov volatile fatty acids (TVFA), acetic acid, butyric acid thiab branched chain volatile fatty acids (BCVFA) nce linearly thaumtributyl glyceridetau ntxiv rau hauv lub substrate. Cov txiaj ntsig ntawm kev sim hauv vivo qhia tau hais tias cov khoom qhuav noj (DMI) thiab pH tus nqi txo qis, thiab cov concentration ntawm TVFA, acetic acid, propionic acid, butyric acid thiab BCVFA nce linearly nrog rau kev ntxiv ntawmtributyl glycerideCov txiaj ntsig ntawm kev sim hauv vivo qhia tau hais tias qhov kev noj cov khoom qhuav (DMI) thiab tus nqi pH txo qis, thiab qhov concentration ntawm TVFA, acetic acid, propionic acid, butyric acid thiab BCVFA nce linearly nrog rau kev ntxiv ntawm tributyl glyceride.

Cov txiaj ntsig ntawm kev sim hauv vivo qhia tau hais tias qhov kev noj cov khoom qhuav (DMI) thiab tus nqi pH txo qis, thiab qhov concentration ntawm TVFA, acetic acid, propionic acid, butyric acid thiab BCVFA nce linearly nrog rau kev ntxiv ntawmtributyl glyceride.

2). Txhim kho qhov kev puas tsuaj ntawm cov as-ham

Qhov pom tseeb ntawm DM, CP, NDF thiab ADF nce linearly thaumtributyl glyceridetau ntxiv rau lub substrate hauv vitro.

3). Txhim kho qhov kev ua haujlwm ntawm cellulose degrading enzyme

Qhov ntxiv ntawmtributyrinHauv vitro, cov kev ua ntawm xylanase, carboxymethyl cellulase thiab microcrystalline cellulase tau nce linearly. Cov kev sim hauv vivo qhia tau tias triglyceride tau nce linearly cov kev ua ntawm xylanase thiab carboxymethyl cellulase.

4). Txhim kho cov protein ntau ntawm cov kab mob me me

Cov kev sim hauv vivo qhia tau tiastributyrinlinearly nce tus nqi txhua hnub ntawm allantoin, uric acid thiab nqus cov microbial purine hauv cov zis, thiab ua rau cov synthesis ntawm rumen microbial nitrogen nce ntxiv.

Xaus lus

Tributyrintxhim kho qhov kev tsim cov protein microbial rumen, cov ntsiab lus ntawm tag nrho cov roj fatty acids thiab cov haujlwm ntawm cellulose degrading enzymes, thiab txhawb kev rhuav tshem thiab kev siv cov khoom qhuav, cov protein nyoos, cov tshuaj ntxuav tsis muaj zog thiab cov tshuaj ntxuav kua qaub hauv cov zaub mov noj.

Nws qhia tau hais tias triglyceride muaj ib qho zoo rau cov qoob loo thiab fermentation ntawm rumen microbial protein, thiab tej zaum yuav muaj ib qho zoo rau kev ua tau zoo ntawm cov yaj laus.