AbstractQhov kev sim tau ua los kawm txog cov teebmeem ntawm diludine rau kev tso qe thiab qhov zoo ntawm qe hauv cov qaib thiab txoj hauv kev rau cov txheej txheem ntawm cov teebmeem los ntawm kev txiav txim siab cov ntsuas ntawm cov qe thiab cov ntshav serum 1024 ROM qaib tau muab faib ua plaub pawg txhua tus suav nrog plaub qhov rov ua dua ntawm 64 qaib txhua tus, Cov pab pawg kho mob tau txais tib yam khoom noj khoom haus basal ntxiv nrog 0, 100, 150, 200 mg / kg diludine feem rau 80 d. Cov txiaj ntsig tau raws li nram no. Ntxiv ntawm diludine rau hauv kev noj zaub mov txhim kho cov qaib tso qe kev ua tau zoo, ntawm qhov kev kho mob 150 mg / kg yog qhov zoo tshaj plaws; nws tus nqi ntawm kev tso qe tau nce los ntawm 11.8% (p < 0.01), qe hloov pauv tau txo qis los ntawm 10.36% (p < 0 01). Qhov hnyav qe tau nce nrog kev nce ntawm diludine ntxiv. Diludine txo qis qhov concentration ntawm uric acid hauv cov ntshav (p < 0.01); ntxiv diludine txo qis qhov serum Ca hauv cov ntshav2+thiab cov ntsiab lus inorganic phosphate, thiab kev ua haujlwm ntawm alkine phosphatase (ALP) ntawm serum (p < 0.05), yog li nws muaj cov teebmeem tseem ceeb rau kev txo qis qe tawg (p < 0.05) thiab qhov tsis zoo (p < 0.05); diludine ua rau qhov siab albumen nce siab. Tus nqi Haugh (p < 0.01), lub plhaub tuab thiab lub plhaub hnyav (p < 0.05), 150 thiab 200mg / kg diludine kuj txo qis tag nrho cov choleasterol hauv qe qe (p < 0 05), tab sis nce qe qe hnyav (p < 0.05). Tsis tas li ntawd, diludine tuaj yeem txhim kho kev ua haujlwm ntawm lipase (p < 0.01), thiab txo cov ntsiab lus ntawm triglyceride (TG3) (p < 0.01) thiab cholesterol (CHL) (p < 0 01) hauv serum, nws txo qis feem pua ntawm cov rog plab (p < 0.01) thiab cov rog siab (p < 0.01), muaj peev xwm tiv thaiv cov qaib los ntawm lub siab rog. Diludine ua rau muaj kev ua haujlwm ntawm SOD hauv cov ntshav ntau ntxiv (p < 0 01) thaum nws tau ntxiv rau hauv kev noj haus rau ntau tshaj 30 hnub. Txawm li cas los xij, tsis muaj qhov sib txawv tseem ceeb hauv kev ua haujlwm ntawm GPT thiab GOT ntawm cov ntshav ntawm pawg tswj thiab pawg kho. Nws tau xav tias diludine tuaj yeem tiv thaiv cov hlwb ntawm cov hlwb los ntawm oxidation.
Cov lus tseem ceebDiludine; qaib; SOD; roj cholesterol; triglyceride, lipase
Lub diludine yog cov tshuaj vitamin tshiab uas tsis muaj txiaj ntsig zoo rau kev tiv thaiv kev laus thiab muaj cov teebmeem[1-3]ntawm kev txwv tsis pub oxidation ntawm cov biological membrane thiab stabilizing cov ntaub so ntswg ntawm cov biological cell, thiab lwm yam. Xyoo 1970, tus kws tshaj lij ua liaj ua teb ntawm Latvia hauv yav dhau los Soviet Union pom tias diludine muaj cov teebmeem[4]ntawm kev txhawb nqa kev loj hlob ntawm cov nqaij qaib thiab tiv taus kev khov thiab kev laus rau qee cov nroj tsuag. Nws tau tshaj tawm tias diludine tsis yog tsuas yog tuaj yeem txhawb kev loj hlob ntawm cov tsiaj, tab sis txhim kho kev ua haujlwm ntawm cov tsiaj txhu thiab txhim kho kev xeeb tub, cov mis nyuj, cov qe thiab cov qe ntawm cov poj niam[1, 2, 5-7]. Kev tshawb nrhiav txog diludine hauv Suav teb tau pib txij li xyoo 1980, thiab feem ntau ntawm cov kev tshawb fawb txog diludine hauv Suav teb tsuas yog siv rau kev siv cov txiaj ntsig xwb, thiab ob peb qhov kev sim ntawm cov noog tso tau tshaj tawm. Chen Jufang (1993) tau tshaj tawm tias diludine tuaj yeem txhim kho cov qe thiab qhov hnyav ntawm cov qe ntawm cov noog, tab sis tsis tau tob zuj zus.[5]kev kawm txog txoj kev ua haujlwm ntawm nws. Yog li ntawd, peb tau siv txoj kev kawm txog qhov cuam tshuam thiab txoj kev ua haujlwm ntawm nws los ntawm kev pub cov qaib tso nrog cov khoom noj uas muaj diludine, thiab ib feem ntawm qhov tshwm sim tam sim no tau tshaj tawm raws li nram no:
Rooj 1 Cov khoom xyaw thiab cov khoom noj muaj txiaj ntsig ntawm kev noj zaub mov sim
%
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Cov khoom noj muaj txiaj ntsig
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Pob kws 62 ME③ 11.97
Taum pauv 20 CP 17.8
Pluas ntses 3 Ca 3.42
Pluas mov rapeseed 5 P 0.75
Pob txha noj 2 M thiab 0.43
Pob zeb hmoov 7.5 M et Cys 0.75
Methionine 0.1
Ntshav 0.3
Cov vitamins ntau yam ① 10
Cov khoom siv tsis muaj kab mob ② 0.1
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① Multivitamin: 11mg ntawm riboflavin, 26mg ntawm folic acid, 44mg ntawm oryzanin, 66mg ntawm niacin, 0.22mg ntawm biotin, 66mg ntawm B6, 17.6ug ntawm B12, 880mg ntawm choline, 30mg ntawm VK, 66IU ntawm VE, 6600ICU ntawm VDthiab 20000ICU ntawm VA, ntxiv rau txhua kilogram ntawm cov khoom noj; thiab 10g multivitamin ntxiv rau txhua 50kg ntawm cov khoom noj.
② Cov ntsiab lus tseem ceeb (mg/kg): 60 mg ntawm Mn, 60mg ntawm Zn, 80mg ntawm Fe, 10mg ntawm Cu, 0.35mg ntawm I thiab 0.3mg ntawm Se raug ntxiv rau txhua kilogram ntawm cov khoom noj.
③ Lub chav ntawm lub zog metabolizable yog hais txog MJ/kg.
1. Cov ntaub ntawv thiab txoj kev
1.1 Cov ntaub ntawv sim
Beijing Sunpu Biochem. & Tech. Co., Ltd. yuav tsum muab cov diludine; thiab tus tsiaj sim yuav tsum yog cov qaib tso qe Roman uas muaj hnub nyoog 300 hnub.
Kev noj zaub mov sim: kev noj zaub mov sim yuav tsum tau npaj raws li qhov xwm txheej tiag tiag thaum lub sijhawm tsim khoom raws li tus qauv NRC, raws li qhia hauv Rooj 1.
1.2 Txoj kev sim
1.2.1 Kev sim pub mis: yuav tsum tau ua qhov kev sim pub mis rau hauv lub teb ua liaj ua teb ntawm Hongji Company hauv Jiande City; yuav tsum xaiv 1024 tus qaib Roman tso qe thiab faib ua plaub pawg random thiab txhua pawg rau 256 daim (txhua pawg yuav tsum rov ua dua plaub zaug, thiab txhua tus qaib yuav tsum rov ua dua 64 zaug); cov qaib yuav tsum tau pub plaub yam khoom noj uas muaj cov ntsiab lus sib txawv ntawm diludine, thiab yuav tsum ntxiv 0, 100, 150, 200mg / kg ntawm cov khoom noj rau txhua pawg. Qhov kev sim tau pib thaum Lub Plaub Hlis 10, 1997; thiab cov qaib tuaj yeem nrhiav zaub mov thiab haus dej tau yooj yim. Cov zaub mov uas txhua pawg noj, qhov nrawm ntawm kev tso qe, cov qe tawg thiab tus lej ntawm cov qe tsis zoo yuav tsum tau sau tseg. Ntxiv mus, qhov kev sim tau xaus rau Lub Rau Hli 30, 1997.
1.2.2 Kev Ntsuas Qhov Zoo ntawm Qe: Yuav tsum tau noj 20 lub qe thaum lub sijhawm sim plaub hnub, plaub caug plaub caug hnub kom ntsuas tau cov cim qhia txog qhov zoo ntawm Qe, xws li cov duab qe, qhov hnyav ntawm lub plhaub, qhov tuab ntawm lub plhaub, qhov ntsuas ntawm lub qe, qhov hnyav ntawm lub qe, thiab lwm yam. Ntxiv mus, cov roj cholesterol hauv lub qe yuav tsum tau ntsuas los ntawm kev siv txoj kev COD-PAP nyob rau hauv qhov muaj Cicheng reagent tsim los ntawm Ningbo Cixi Biochemical Test Plant.
1.2.3 Kev Ntsuas Cov Qib Biochemical Index: Yuav tsum coj 16 tus qaib kuaj los ntawm txhua pawg txhua zaus thaum qhov kev kuaj tau siv rau 30 hnub thiab thaum qhov kev kuaj tiav lawm los npaj cov serum tom qab kuaj cov ntshav los ntawm cov leeg ntawm tis. Cov serum yuav tsum khaws cia rau qhov kub qis (-20 ℃) txhawm rau ntsuas cov qib biochemical. Yuav tsum ntsuas feem pua ntawm cov rog hauv plab thiab cov roj hauv siab tom qab tua thiab rho tawm cov rog hauv plab thiab lub siab thaum ua tiav qhov kev kuaj ntshav.
Yuav tsum ntsuas cov superoxide dismutase (SOD) los ntawm kev siv txoj kev saturation thaum muaj cov khoom siv reagent uas tsim los ntawm Beijing Huaqing Biochem. & Tech. Research Institute. Yuav tsum ntsuas cov uric acid (UN) hauv cov ntshav los ntawm kev siv txoj kev Uricase-PAP thaum muaj cov khoom siv reagent Cicheng; yuav tsum ntsuas cov triglyceride (TG3) los ntawm kev siv txoj kev GPO-PAP ib kauj ruam thaum muaj cov khoom siv reagent Cicheng; yuav tsum ntsuas cov lipase los ntawm kev siv nephelometry thaum muaj cov khoom siv reagent Cicheng; yuav tsum ntsuas cov roj cholesterol tag nrho hauv cov ntshav (CHL) los ntawm kev siv txoj kev COD-PAP thaum muaj cov khoom siv reagent Cicheng; yuav tsum ntsuas cov glutamic-pyruvic transaminase (GPT) los ntawm kev siv colorimetry thaum muaj cov khoom siv reagent Cicheng; yuav tsum ntsuas cov glutamic-oxalacetic transaminase (GOT) los ntawm kev siv colorimetry thaum muaj cov khoom siv reagent Cicheng; yuav tsum ntsuas alkaline phosphatase (ALP) los ntawm kev siv txoj kev ntsuas tus nqi thaum muaj Cicheng reagent kit; cov calcium ion (Ca2+) hauv cov ntshav yuav tsum tau ntsuas los ntawm kev siv txoj kev methylthymol xiav complexone thaum muaj Cicheng reagent kit; cov inorganic phosphorus (P) yuav tsum tau ntsuas los ntawm kev siv txoj kev molybdate xiav thaum muaj Cicheng reagent kit.
2 Cov txiaj ntsig ntawm kev xeem
2.1 Cov nyhuv rau kev ua haujlwm ntawm kev tso
Cov kev ua tau zoo ntawm cov pab pawg sib txawv uas tau ua los ntawm kev siv diludine tau qhia nyob rau hauv Rooj 2.
Rooj 2 Kev Ua Tau Zoo ntawm cov qaib uas pub nrog cov khoom noj khoom haus ntxiv uas muaj plaub theem ntawm diludine
| Tus nqi ntawm diludine uas yuav tsum ntxiv (mg/kg) | ||||
| 0 | 100 | 150 | 200 | |
| Kev noj zaub mov (g) |  | |||
| Tus nqi tso (%) | ||||
| Qhov hnyav nruab nrab ntawm qe (g) | ||||
| Qhov sib piv ntawm cov khoom siv rau qe | ||||
| Tus nqi qe tawg (%) | ||||
| Tus nqi ntawm cov qe tsis zoo (%) | ||||
Los ntawm Rooj 2, cov nqi tso ntawm txhua pawg uas ua los ntawm kev siv diludine tau zoo dua pom tseeb, qhov twg qhov cuam tshuam thaum ua los ntawm kev siv 150mg / kg yog qhov zoo tshaj plaws (txog li 83.36%), thiab 11.03% (p <0.01) tau zoo dua piv rau pawg siv; yog li ntawd diludine muaj cov txiaj ntsig ntawm kev txhim kho tus nqi tso. Pom los ntawm qhov hnyav nruab nrab ntawm qe, qhov hnyav ntawm qe nce ntxiv (p> 0.05) nrog rau kev nce diludine hauv cov zaub mov txhua hnub. Piv nrog pawg siv, qhov sib txawv ntawm txhua qhov ua tiav ntawm cov pab pawg uas ua los ntawm kev siv 200mg / kg ntawm diludine tsis pom tseeb thaum ntxiv 1.79g ntawm cov zaub mov noj nruab nrab; txawm li cas los xij, qhov sib txawv maj mam pom tseeb nrog rau qhov nce ntxiv ntawm diludine, thiab qhov sib txawv ntawm qhov sib piv ntawm cov khoom siv rau qe ntawm cov khoom ua tiav yog qhov pom tseeb (p <0.05), thiab qhov cuam tshuam zoo tshaj plaws thaum 150mg / kg ntawm diludine thiab yog 1.25: 1 uas txo qis rau 10.36% (p <0.01) piv rau pawg siv. Pom los ntawm qhov nrawm ntawm qe tawg ntawm txhua qhov ua tiav, qhov nrawm ntawm qe tawg (p <0.05) tuaj yeem txo qis thaum diludine ntxiv rau hauv cov khoom noj txhua hnub; thiab feem pua ntawm cov qe tsis zoo raug txo qis (p <0.05) nrog rau kev nce ntxiv ntawm diludine.
2.2 Cov nyhuv rau qhov zoo ntawm qe
Pom los ntawm Rooj 3, qhov ntsuas ntawm cov qe thiab qhov hnyav tshwj xeeb ntawm qe tsis cuam tshuam (p> 0.05) thaum ntxiv diludine rau hauv cov zaub mov txhua hnub, thiab qhov hnyav ntawm lub plhaub nce ntxiv raws li kev nce diludine ntxiv rau hauv cov zaub mov txhua hnub, qhov twg qhov hnyav ntawm lub plhaub nce rau 10.58% thiab 10.85% (p < 0.05) feem piv rau cov pab pawg siv thaum ntxiv 150 thiab 200mg / kg ntawm diludine; qhov tuab ntawm lub plhaub qe nce ntxiv raws li kev nce diludine hauv cov zaub mov txhua hnub, qhov twg qhov tuab ntawm lub plhaub qe nce rau 13.89% (p < 0.05) thaum ntxiv 100mg / kg ntawm diludine piv rau cov pab pawg siv, thiab qhov tuab ntawm lub plhaub qe nce rau 19.44% (p < 0.01) thiab 27.7% (p < 0.01) feem thaum ntxiv 150 thiab 200mg / kg. Lub Haugh unit (p<0.01) tau zoo dua thaum ntxiv diludine, uas qhia tau tias diludine muaj cov txiaj ntsig ntawm kev txhawb nqa kev tsim cov albumen tuab ntawm qe dawb. Diludine muaj lub luag haujlwm ntawm kev txhim kho cov ntsuas ntawm qe daj, tab sis qhov sib txawv tsis yog qhov tseeb (p<0.05). Cov ntsiab lus ntawm cov roj cholesterol ntawm qe daj ntawm txhua pawg yog qhov sib txawv thiab tuaj yeem txo qis (p<0.05) tom qab ntxiv 150 thiab 200mg/kg ntawm diludine. Qhov hnyav sib piv ntawm qe daj sib txawv ntawm ib leeg vim muaj ntau yam diludine ntxiv, qhov twg qhov hnyav sib piv ntawm qe daj tau zoo dua rau 18.01% thiab 14.92% (p<0.05) thaum 150mg/kg thiab 200mg/kg piv rau pawg siv; yog li ntawd, diludine tsim nyog muaj cov txiaj ntsig ntawm kev txhawb nqa kev tsim cov qe daj.
Rooj 3 Cov teebmeem ntawm diludine rau qhov zoo ntawm qe
| Tus nqi ntawm diludine uas yuav tsum ntxiv (mg/kg) | ||||
| Qe zoo | 0 | 100 | 150 | 200 |
| Cov duab qe (%) |  | |||
| Lub zog tshwj xeeb ntawm qe (g/cm3) | ||||
| Qhov hnyav sib piv ntawm lub plhaub qe (%) | ||||
| Thickness ntawm qe plhaub (hli) | ||||
| Chav tsev loj (U) | ||||
| Cov ntsuas qe qe (%) | ||||
| Cov roj cholesterol ntawm qe qe (%) | ||||
| Qhov hnyav sib piv ntawm qe qe (%) | ||||
2.3 Cov teebmeem rau feem pua ntawm cov rog hauv plab thiab cov ntsiab lus ntawm cov rog siab ntawm cov qaib tso qe
Saib Daim Duab 1 thiab Daim Duab 2 rau cov teebmeem ntawm diludine rau feem pua ntawm cov rog hauv plab thiab cov ntsiab lus ntawm cov rog siab ntawm cov qaib tso qe.
Daim Duab 1 Cov nyhuv ntawm diludine rau feem pua ntawm cov rog hauv lub cev (PAF) ntawm cov qaib tso qe
| Feem pua ntawm cov rog hauv lub cev | |
| Tus nqi ntawm diludine uas yuav tsum ntxiv |
Daim Duab 2 Cov nyhuv ntawm diludine rau cov rog hauv siab (LF) ntawm cov qaib tso qe
| Cov rog hauv siab | |
| Tus nqi ntawm diludine uas yuav tsum ntxiv |
Pom los ntawm Daim Duab 1, feem pua ntawm cov rog hauv plab ntawm pawg neeg sim raug txo rau 8.3% thiab 12.11% (p < 0.05) feem ntau thaum 100 thiab 150mg / kg ntawm diludine piv rau pawg siv, thiab feem pua ntawm cov rog hauv plab raug txo rau 33.49% (p < 0.01) thaum ntxiv 200mg / kg ntawm diludine. Pom los ntawm Daim Duab 2, cov rog hauv siab (qhuav kiag li) ua tiav los ntawm 100, 150, 200mg / kg ntawm diludine feem ntau raug txo rau 15.00% (p < 0.05), 15.62% (p < 0.05) thiab 27.7% (p < 0.01) feem ntau piv rau pawg siv; yog li ntawd, diludine muaj cov nyhuv ntawm kev txo cov feem pua ntawm cov rog hauv plab thiab cov rog hauv siab ntawm cov ntsiab lus tso, qhov twg cov nyhuv zoo tshaj plaws thaum ntxiv 200mg / kg ntawm diludine.
2.4 Cov nyhuv rau cov ntsuas biochemical hauv cov ntshav
Pom los ntawm Rooj 4, qhov sib txawv ntawm cov khoom ua tiav thaum lub sijhawm Theem I (30d) ntawm SOD xeem tsis pom tseeb, thiab cov ntsuas biochemical ntawm txhua pawg uas diludine ntxiv rau hauv Theem II (80d) ntawm qhov kev xeem siab dua li pawg siv (p < 0.05). Cov uric acid (p < 0.05) hauv cov ntshav tuaj yeem txo qis thaum ntxiv 150mg / kg thiab 200mg / kg ntawm diludine; thaum cov nyhuv (p < 0.05) muaj thaum ntxiv 100mg / kg ntawm diludine hauv Theem I. Diludine tuaj yeem txo cov triglyceride hauv cov ntshav, qhov twg cov nyhuv zoo tshaj plaws (p < 0.01) hauv pawg thaum ntxiv 150mg / kg ntawm diludine hauv Theem I, thiab zoo tshaj plaws hauv pawg thaum ntxiv 200mg / kg ntawm diludine hauv Theem II. Tag nrho cov roj cholesterol hauv cov ntshav tau txo qis raws li kev nce ntxiv ntawm diludine ntxiv rau hauv cov zaub mov txhua hnub, tshwj xeeb tshaj yog cov ntsiab lus ntawm tag nrho cov roj cholesterol hauv cov ntshav tau txo qis rau 36.36% (p < 0.01) thiab 40.74% (p < 0.01) feem thaum 150mg / kg thiab 200mg / kg ntawm diludine ntxiv rau hauv Theem I piv nrog pawg siv, thiab txo qis rau 26.60% (p < 0.01), 37.40% (p < 0.01) thiab 46.66% (p < 0.01) feem thaum 100mg / kg, 150mg / kg thiab 200mg / kg ntawm diludine ntxiv rau hauv Theem II piv nrog pawg siv. Ntxiv mus, ALP nce ntxiv raws li kev nce ntxiv ntawm diludine ntxiv rau hauv cov zaub mov txhua hnub, thaum cov nqi ntawm ALP hauv pawg uas ntxiv 150mg / kg thiab 200mg / kg ntawm diludine siab dua li pawg siv (p <0.05) pom tseeb.
Rooj 4 Cov teebmeem ntawm diludine rau cov qauv serum
| Tus nqi ntawm diludine uas yuav ntxiv (mg/kg) hauv Theem I (30d) ntawm kev sim | ||||
| Khoom | 0 | 100 | 150 | 200 |
| Superoxide dismutase (mg/mL) |  | |||
| Cov kua qaub uric | ||||
| Triglycerides (mmol/L) | ||||
| Lipase (U/L) | ||||
| Cov roj cholesterol (mg/dL) | ||||
| Glutamic-pyruvic transaminase (U/L) | ||||
| Glutamic-oxalacetic transaminase (U/L) | ||||
| Alkaline phosphatase (mmol/L) | ||||
| Calcium ion (mmol/L) | ||||
| Cov phosphorus uas tsis muaj organic (mg/dL) | ||||
| Tus nqi ntawm diludine uas yuav ntxiv (mg/kg) hauv Theem II (80d) ntawm kev sim | ||||
| Khoom | 0 | 100 | 150 | 200 |
| Superoxide dismutase (mg/mL) |  | |||
| Cov kua qaub uric | ||||
| Triglycerides (mmol/L) | ||||
| Lipase (U/L) | ||||
| Cov roj cholesterol (mg/dL) | ||||
| Glutamic-pyruvic transaminase (U/L) | ||||
| Glutamic-oxalacetic transaminase (U/L) | ||||
| Alkaline phosphatase (mmol/L) | ||||
| Calcium ion (mmol/L) | ||||
| Cov phosphorus uas tsis muaj organic (mg/dL) | ||||
3 Kev tshuaj xyuas thiab kev sib tham
3.1 Cov diludine hauv qhov kev sim tau txhim kho qhov nrawm ntawm kev tso qe, qhov hnyav ntawm qe, Haugh unit thiab qhov hnyav sib piv ntawm cov qe yolk, uas qhia tau tias cov diludine muaj cov txiaj ntsig ntawm kev txhawb nqa kev sib xyaw ntawm cov protein thiab txhim kho qhov ntau ntawm kev tsim cov protein tuab ntawm qe dawb thiab cov protein ntawm qe yolk. Ntxiv mus, cov ntsiab lus ntawm uric acid hauv cov ntshav tau txo qis; thiab nws feem ntau lees paub tias kev txo qis ntawm cov ntsiab lus ntawm cov nitrogen uas tsis yog protein hauv cov ntshav txhais tau tias qhov ceev ntawm kev zom zaub mov ntawm cov protein tau txo qis, thiab lub sijhawm khaws cia ntawm nitrogen tau ncua. Qhov tshwm sim no tau muab lub hauv paus ntawm kev nce cov protein khaws cia, txhawb kev tso qe thiab txhim kho qhov hnyav ntawm cov qe ntawm cov qaib tso. Qhov tshwm sim ntawm qhov kev sim tau taw qhia tias qhov cuam tshuam ntawm kev tso qe yog qhov zoo tshaj plaws thaum ntxiv 150mg / kg ntawm diludine, uas yog qhov sib xws nrog qhov tshwm sim.[6,7]ntawm Bao Erqing thiab Qin Shangzhi thiab tau txais los ntawm kev ntxiv diludine thaum lub sijhawm lig ntawm cov qaib tso. Cov nyhuv tau txo qis thaum cov diludine ntau tshaj 150mg / kg, uas tej zaum yuav yog vim cov protein hloov pauv[8]tau raug cuam tshuam vim yog kev noj tshuaj ntau dhau thiab kev thauj khoom ntau dhau ntawm cov metabolism ntawm lub cev rau diludine.
3.2 Qhov concentration ntawm Ca2+hauv cov ntshav ntawm cov qe tso tau txo qis, P hauv cov ntshav tau txo qis thaum pib thiab ALP kev ua haujlwm tau nce ntxiv pom tseeb hauv qhov muaj diludine, uas qhia tau tias diludine cuam tshuam rau kev ua haujlwm ntawm Ca thiab P pom tseeb. Yue Wenbin tau tshaj tawm tias diludine tuaj yeem txhawb kev nqus.[9] ntawm cov ntsiab lus ntxhia Fe thiab Zn; ALP feem ntau muaj nyob rau hauv cov ntaub so ntswg, xws li daim siab, pob txha, txoj hnyuv, lub raum, thiab lwm yam; ALP hauv cov ntshav yog los ntawm daim siab thiab pob txha feem ntau; ALP hauv pob txha muaj nyob rau hauv osteoblast feem ntau thiab tuaj yeem sib xyaw cov phosphate ion nrog Ca2 los ntawm cov ntshav tom qab hloov pauv los ntawm kev txhawb nqa kev rhuav tshem ntawm phosphate thiab nce qhov concentration ntawm phosphate ion, thiab tau tso rau ntawm pob txha hauv daim ntawv ntawm hydroxyapatite, thiab lwm yam kom ua rau txo qis ntawm Ca thiab P hauv cov ntshav, uas yog sib xws nrog kev nce ntawm cov qe plhaub tuab thiab qhov hnyav ntawm cov qe plhaub hauv cov cim qhia zoo ntawm qe. Ntxiv mus, qhov ceev ntawm cov qe tawg thiab feem pua ntawm cov qe tsis zoo tau txo qis pom tseeb hauv cov nqe lus ntawm kev ua haujlwm tso, uas kuj piav qhia txog qhov no.
3.3 Qhov kev tso rog hauv plab thiab cov rog hauv siab ntawm cov qaib tso tau txo qis los ntawm kev ntxiv diludine rau hauv cov zaub mov noj, uas qhia tau tias diludine muaj cov nyhuv ntawm kev txwv tsis pub tsim cov rog hauv lub cev. Ntxiv mus, diludine tuaj yeem txhim kho kev ua haujlwm ntawm lipase hauv cov ntshav thaum ntxov; qhov kev ua haujlwm ntawm lipase tau nce ntxiv pom tseeb hauv pawg uas 100mg / kg ntawm diludine tau ntxiv, thiab cov ntsiab lus ntawm triglyceride thiab cov roj cholesterol hauv cov ntshav tau txo qis (p <0.01), uas qhia tau tias diludine tuaj yeem txhawb kev rhuav tshem ntawm triglyceride thiab txwv tsis pub tsim cov roj cholesterol. Qhov kev tso rog tuaj yeem raug txwv vim tias cov enzyme ntawm lipid metabolism hauv lub siab[10,11], thiab txo cov roj cholesterol hauv qe qe kuj piav qhia txog qhov no [13]. Chen Jufang tau tshaj tawm tias diludine tuaj yeem tiv thaiv kev tsim cov rog hauv tus tsiaj thiab txhim kho cov nqaij ntshiv ntawm cov broilers thiab npua, thiab muaj cov txiaj ntsig ntawm kev kho mob siab rog. Qhov tshwm sim ntawm qhov kev sim tau piav qhia txog cov txheej txheem ntawm kev ua haujlwm no, thiab cov txiaj ntsig ntawm kev txiav thiab kev soj ntsuam ntawm cov qaib kuaj kuj tau ua pov thawj tias diludine tuaj yeem txo qhov tshwm sim ntawm siab rog ntawm cov qaib tso qe.
3.4 GPT thiab GOT yog ob qho cim qhia tseem ceeb uas qhia txog kev ua haujlwm ntawm lub siab thiab lub plawv, thiab lub siab thiab lub plawv yuav raug puas tsuaj yog tias cov haujlwm ntawm nws siab dhau. Cov haujlwm ntawm GPT thiab GOT hauv cov ntshav tsis tau hloov pauv meej thaum ntxiv diludine rau hauv qhov kev xeem, uas qhia tau tias lub siab thiab lub plawv tsis raug puas tsuaj; ntxiv mus, qhov ntsuas ntawm SOD qhia tau tias cov haujlwm ntawm SOD hauv cov ntshav tuaj yeem txhim kho meej thaum siv diludine rau ib lub sijhawm. SOD hais txog qhov tseem ceeb ntawm cov superoxide free radical hauv lub cev; nws yog qhov tseem ceeb rau kev tswj hwm kev ncaj ncees ntawm cov kab mob hauv lub cev, txhim kho lub cev tiv thaiv kab mob thiab tswj kev noj qab haus huv ntawm tus tsiaj thaum cov ntsiab lus ntawm SOD hauv lub cev nce ntxiv. Quh Hai, thiab lwm yam. tau tshaj tawm tias diludine tuaj yeem txhim kho cov haujlwm ntawm 6-glucose phosphate dehydrogenase hauv cov kab mob hauv lub cev thiab ua kom cov ntaub so ntswg ruaj khov [2] ntawm cov kab mob hauv lub cev. Sniedze tau taw qhia tias diludine tau txwv tsis pub ua haujlwm [4] ntawm NADPH cytochrome C reductase pom tseeb tom qab kawm txog kev sib raug zoo ntawm diludine thiab cov enzyme tseem ceeb hauv NADPH cov saw hlau hloov pauv hluav taws xob hauv nas lub siab microsome. Odydents kuj tau taw qhia tias diludine muaj feem cuam tshuam [4] rau lub kaw lus sib xyaw oxidase thiab microsomal enzyme cuam tshuam nrog NADPH; thiab lub tshuab ua haujlwm ntawm diludine tom qab nkag mus rau hauv tsiaj yog ua lub luag haujlwm ntawm kev tiv thaiv oxidation thiab tiv thaiv cov kab mob hauv lub cev [8] los ntawm kev cuam tshuam cov haujlwm ntawm cov enzyme hloov pauv hluav taws xob NADPH ntawm microsome thiab txwv tsis pub ua cov txheej txheem peroxidation ntawm cov lipid compound. Cov txiaj ntsig ntawm kev xeem tau ua pov thawj tias kev tiv thaiv ntawm diludine rau cov kab mob hauv lub cev los ntawm kev hloov pauv ntawm SOD kev ua haujlwm rau kev hloov pauv ntawm GPT thiab GOT thiab tau ua pov thawj cov txiaj ntsig ntawm kev tshawb fawb ntawm Sniedze thiab Odydents.
Siv ua piv txwv
1 Zhou Kai, Zhou Mingjie, Qin Zhongzhi, thiab lwm yam. Kev tshawb fawb txog diludine ntawm kev txhim kho kev ua haujlwm ntawm cov yajJ. Cov nyom thiabLivestock 1994 (2): 16-17
2 Qu Hai, Lv Ye, Wang Baosheng, Cov nyhuv ntawm diludine ntxiv rau hauv kev noj haus txhua hnub rau kev xeeb tub thiab cov phev zoo ntawm cov nqaij luav.J. Phau Ntawv Xov Xwm Suav Txog Kev Ua Liaj Ua Teb Luav1994(6): 6-7
3 Chen Jufang, Yin Yuejin, Liu Wanhan, thiab lwm yam. Kev sim ntawm kev siv diludine ua cov khoom noj khoom haus ntxivKev Tshawb Fawb Txog Kev Pub Noj1993 (3): 2-4
4 Zheng Xiaozhong, Li Kelu, Yue Wenbin, thiab lwm yam. Kev sib tham txog kev siv thiab cov txheej txheem ntawm kev ua haujlwm ntawm diludine ua tus txhawb kev loj hlob ntawm cov qaibKev Tshawb Fawb Txog Kev Pub Noj1995 (7): 12-13
5 Chen Jufang, Yin Yuejin, Liu Wanhan, thiab lwm yam. Kev sim ntawm kev siv diludine ua cov khoom noj khoom haus ntxivKev Tshawb Fawb Txog Kev Pub Noj1993 (3): 2-5
6 Bao Erqing, Gao Baohua, Kev Ntsuas ntawm diludine rau kev pub mis yug Peking osKev Tshawb Fawb Txog Kev Pub Noj1992 (7): 7-8
7 Qin Shangzhi Kev Ntsuas ntawm kev txhim kho kev tsim khoom ntawm cov qaib nqaij thaum lub sijhawm kawg ntawm kev tso qe los ntawm kev siv diludineGuangxi Journal of Animal Husbandry & Veterinary Medicine1993.9(2): 26-27
8 Dibner J Jl Lvey FJ Cov protein hauv siab thiab cov amino acid metabolian hauv cov nqaij qaib Kev Tshawb Fawb Txog Nqaij Qaib1990.69(7): 1188-1194
9 Yue Wenbin, Zhang Jianhong, Zhao Peie, thiab lwm yam. Kev kawm txog kev ntxiv cov tshuaj diludine thiab Fe-Zn rau hauv cov khoom noj txhua hnub ntawm cov qaib tso qePub thiab Tsiaj Txhu1997, 18(7): 29-30
10 Mildner A na M, Steven D Clarke Porcine fatty acid synthase cloning ntawm ib qho DNA sib txuas, kev faib cov ntaub so ntswg ntawm itsmRNA thiab kev txwv tsis pub muaj kev qhia tawm los ntawm somatotropin thiab cov protein noj haus J Nutri 1991, 121 900
11 W alzon RL Smon C, M orishita T, et a I Fatty liver hemorrhagic syndrome hauv cov qaib noj ntau dhau cov zaub mov huv Cov haujlwm enzyme xaiv thiab daim siab histology hauv kev sib raug zoo rau daim siab honorrhage thiab resproductive performanceKev Tshawb Fawb Txog Nqaij Qaib,1993 72(8): 1479-1491
12 Donaldson WE Lipid metabolism hauv daim siab ntawm cov qaib teb rau kev pub misKev Tshawb Fawb Txog Nqaij Qaib. 1990, 69(7): 1183-1187
13 Ksiazk ieu icz J. K ontecka H, H ogcw sk i L Ib daim ntawv qhia txog cov roj cholesterol hauv cov ntshav ua ib qho cim qhia txog kev rog hauv cov osPhau ntawv Journal of Aninal thiab Feed Science,1992, 1(3/4): 289-294
Lub sijhawm tshaj tawm: Lub Rau Hli-07-2021
